...ONWARD and UPWARD!
Brilacidin is showing expanded utility in the "non-enveloped virus" quadrant...with successful and expanded studies...
IMO "several" Gov/University labs are ALL OVER BRILACIDIN...
With several "compassionate use" cases...under Brilacidin's belt...it is obvious there are Clinicians who have heard of Brilacidin/Covid and found it worth the risk of use...look for them to spread any word of improvement...(IMO there likely was...if the first struck out...there likely would not have been a second or third...a tea leaves read.
My personal hope is that IPIX takes all the time it requires to assemble and analyze all the data...IMO the longer it takes, the more thorough the results...
Meanwhile, a base is being solidly established...but more importantly...more VIA SEVERAL GOV. LABORATORIES...DOCUMENTATION OF BRILACIDIN'S ROBUST RESPONSE IN LABS TO ALL VIRUSES...will be PUBLISHED....and Dr DeGrado's genius will be recognized, and longs rewarded...
IPIX and most Biotechs...have Never had a more acclaimed SCIENTIST who DEVELOPED/DISCOVERED Brilacidin (owned by IPIX), as SCIENTIFIC ADVISOR supporting the Clinical Trials process for FDA approval.
IMO Dr. Kessler (a straight shooter) KNOW HIM AND IT...and is one who is watching very closely to these Trials and Compassionate Use cases!
Read on...
William F. "Bill" DeGrado, Ph.D., is the Professor of Pharmaceutical Chemistry at the University of California, San Francisco (UCSF) [1] and a member of the National Academy of Sciences.
He received a B.S. (chemistry) from Kalamazoo College and a Ph.D. (Chemistry) from the University of Chicago in 1977 working with Emil T. Kaiser and F. Kezdy. His graduate work focused on the design of the oxime resin for solid-phase synthesis,[2] which was used for synthesis of protected peptides and is still in use for various types of combinatorial chemistry today. He also used peptide design to demonstrate that melittin adopts an amphiphilic helical structure,[3] which is responsible for its membrane-disrupting activity.
He first held an industrial position at DuPont Central Research & Development (later DuPont Merck Pharmaceutical Company). He transitioned to academia in 1996, joining the University of Pennsylvania as the George W. Raiziss professor of biochemistry and biophysics and then moved to UCSF in 2011.
His published research includes contributions to the fields of protein design, synthesis of peptidomimetics, and characterization of membrane-active peptides and proteins, most notably the M2 protein.
The M2 proton channel from Influenza A virus. DeGrado’s early work with the groups of Robert Lamb and Larry Pinto established the overall structure and mechanism of the M2 proton channel,[4] which is the target of the anti-influenza drugs, amantadine and rimantadine. A decade later their crystallographic,[5][6] and NMR structures[7][8] defined the fine details of the binding site for these drugs and explained the mechanism of the growing problem of amantadine-resistance. With Michael Klein, Robert Lamb and Larry Pinto, DeGrado extensively characterized the physiological properties of many drug-resistant mutants of the channel, identified those most likely to lead to resistance, and designed new drugs to address the problem of drug-resistant forms of influenza A virus.[7][9]
Awards
1988 Du Vigneaud Award for Young Investigators in Peptide Research
1989 Protein Society Young Investigator Award
1992 Eli Lilly Award in Biological Chemistry
2005 The American Peptide Society Merrifield Award
2008 The ACS Ralph F. Hirschmann Award in Peptide Chemistry
2015 The Stein and Moore Award
2018 American Chemical Society Murray Goodman Memorial Prize[10]
References
"William Degrado, PhD". UCSF. Retrieved 18 January 2014.
"DeGrado, W. F., and Kaiser, E. T. J. Org. Chem. 40, 1295-1230". 1980.
"DeGrado, W. F., Kèzdy, F. J., and Kaiser, E. T. Design, synthesis, and characterization of a cytotoxic peptide with melittin-like activity, J. Am. Chem. Soc. 103, 679-681". 1981.
"Pinto, L. H., Dieckmann, G. R., Gandhi, C. S., Papworth, C. G., Braman, J., Shaughnessy, M. A., Lear, J. D., Lamb, R. A., and DeGrado, W. F. A functionally defined model for the M2 proton channel of influenza A virus suggests a mechanism for its ion selectivity, Proc. Natl. Acad. Sci. U.S.A. 94, 11301-11306". 1997.
"Stouffer, A. L., Acharya, R., Salom, D., Levine, A. S., Di Costanzo, L., Soto, C. S., Tereshko, V., Nanda, V., Stayrook, S., and DeGrado, W. F. Structural basis for the function and inhibition of an influenza virus proton channel, Nature 451, 596-599". 2008.
"Acharya, R., Carnevale, V., Fiorin, G., Levine, B. G., Polishchuk, A. L., Balannik, V., Samish, I., Lamb, R. A., Pinto, L. H., DeGrado, W. F., and Klein, M. L. Structure and mechanism of proton transport through the transmembrane tetrameric M2 protein bundle of the influenza A virus, Proc Natl Acad Sci U S A 107, 15075-15080". 2010.
"Wang, J., Wu, Y., Ma, C., Fiorin, G., Wang, J., Pinto, L. H., Lamb, R. A., Klein, M. L., and DeGrado, W. F. Structure and inhibition of the drug-resistant S31N mutant of the M2 ion channel of influenza A virus, Proc. Natl. Acad. Sci. U.S.A. 110, 1315-1320". 2013.
"Cady, S. D., Schmidt-Rohr, K., Wang, J., Soto, C. S., DeGrado, W. F., and Hong, M. Structure of the amantadine binding site of influenza M2 proton channels in lipid bilayers, Nature 463, 689-692". 2010.
"Wang, J., Ma, C., Fiorin, G., Carnevale, V., Wang, T., Hu, F., Lamb, R. A., Pinto, L. H., Hong, M., Klein, M. L., and DeGrado, W. F. Molecular dynamics simulation directed rational design of inhibitors targeting drug-resistant mutants of influenza A virus M2, J Am Chem Soc 133, 12834-12841". 2011.
"Recipients | ACS Division of Biological Chemistry Website". www.divbiolchem.org. Retrieved 2018-03-22.
External links
Web site of the DeGrado laboratory
PUBLISHED RESEARCH ARTICLE
418 results ...SOME BELOW:
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Actionable Activating Oncogenic ERBB2/HER2 Transmembrane and Juxtamembrane Domain Mutations.
Pahuja KB, Nguyen TT, Jaiswal BS, Prabhash K, Thaker TM, Senger K, Chaudhuri S, Kljavin NM, Antony A, Phalke S, Kumar P, Mravic M, Stawiski EW, Vargas D, Durinck S, Gupta R, Khanna-Gupta A, Trabucco SE, Sokol ES, Hartmaier RJ, Singh A, Chougule A, Trivedi V, Dutt A, Patil V, Joshi A, Noronha V, Ziai J, Banavali SD, Ramprasad V, DeGrado WF, Bueno R, Jura N, Seshagiri S.
Cancer Cell. 2018 Nov 12;34(5):792-806.e5. doi: 10.1016/j.ccell.2018.09.010. Epub 2018 Oct 25.
PMID: 30449325 Free PMC article.
2
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The avß1 integrin plays a critical in vivo role in tissue fibrosis.
Reed NI, Jo H, Chen C, Tsujino K, Arnold TD, DeGrado WF, Sheppard D.
Sci Transl Med. 2015 May 20;7(288):288ra79. doi: 10.1126/scitranslmed.aaa5094.
PMID: 25995225 Free PMC article.
3
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De novo protein design, a retrospective.
Korendovych IV, DeGrado WF.
Q Rev Biophys. 2020 Feb 11;53:e3. doi: 10.1017/S0033583519000131.
PMID: 32041676 Free PMC article. Review.
4
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Prion biology: implications for Alzheimer's disease therapeutics.
Condello C, DeGrado WF, Prusiner SB.
Lancet Neurol. 2020 Oct;19(10):802-803. doi: 10.1016/S1474-4422(20)30274-X. Epub 2020 Sep 16.
PMID: 32949533 No abstract available.
5
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Dual antagonists of a5ß1/avß1 integrin for airway hyperresponsiveness.
Sundaram A, Chen C, Isik Reed N, Liu S, Ki Yeon S, McIntosh J, Tang YZ, Yang H, Adler M, Beresis R, Seiple IB, Sheppard D, DeGrado WF, Jo H.
Bioorg Med Chem Lett. 2020 Nov 15;30(22):127578. doi: 10.1016/j.bmcl.2020.127578. Epub 2020 Sep 29.
PMID: 33007395
6
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Platform to Discover Protease-Activated Antibiotics and Application to Siderophore-Antibiotic Conjugates.
Boyce JH, Dang B, Ary B, Edmondson Q, Craik CS, DeGrado WF, Seiple IB.
J Am Chem Soc. 2020 Dec 23;142(51):21310-21321. doi: 10.1021/jacs.0c06987. Epub 2020 Dec 10.
PMID: 33301681 Free PMC article.
7
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Allosteric cooperation in a de novo-designed two-domain protein.
Pirro F, Schmidt N, Lincoff J, Widel ZX, Polizzi NF, Liu L, Therien MJ, Grabe M, Chino M, Lombardi A, DeGrado WF.
Proc Natl Acad Sci U S A. 2020 Dec 29;117(52):33246-33253. doi: 10.1073/pnas.2017062117. Epub 2020 Dec 14.
PMID: 33318174 Free PMC article.
8
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Deep mutational scanning reveals the structural basis for a-synuclein activity.
Newberry RW, Leong JT, Chow ED, Kampmann M, DeGrado WF.
Nat Chem Biol. 2020 Jun;16(6):653-659. doi: 10.1038/s41589-020-0480-6. Epub 2020 Mar 9.
PMID: 32152544 Free PMC article.
9
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Catalytic efficiency of designed catalytic proteins.
Korendovych IV, DeGrado WF.
Curr Opin Struct Biol. 2014 Aug;27:113-21. doi: 10.1016/j.sbi.2014.06.006. Epub 2014 Jul 19.
PMID: 25048695 Free PMC article. Review.
10
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SNAC-tag for sequence-specific chemical protein cleavage.
Dang B, Mravic M, Hu H, Schmidt N, Mensa B, DeGrado WF.
Nat Methods. 2019 Apr;16(4):319-322. doi: 10.1038/s41592-019-0357-3. Epub 2019 Mar 25.
PMID: 30923372 Free PMC article.
Page 2
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A defined structural unit enables de novo design of small-molecule-binding proteins.
Polizzi NF, DeGrado WF.
Science. 2020 Sep 4;369(6508):1227-1233. doi: 10.1126/science.abb8330.
PMID: 32883865 Free PMC article.
12
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Signal transduction in histidine kinases: insights from new structures.
Bhate MP, Molnar KS, Goulian M, DeGrado WF.
Structure. 2015 Jun 2;23(6):981-94. doi: 10.1016/j.str.2015.04.002. Epub 2015 May 14.
PMID: 25982528 Free PMC article. Review.
13
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Robust Sequence Determinants of a-Synuclein Toxicity in Yeast Implicate Membrane Binding.
Newberry RW, Arhar T, Costello J, Hartoularos GC, Maxwell AM, Naing ZZC, Pittman M, Reddy NR, Schwarz DMC, Wassarman DR, Wu TS, Barrero D, Caggiano C, Catching A, Cavazos TB, Estes LS, Faust B, Fink EA, Goldman MA, Gomez YK, Gordon MG, Gunsalus LM, Hoppe N, Jaime-Garza M, Johnson MC, Jones MG, Kung AF, Lopez KE, Lumpe J, Martyn C, McCarthy EE, Miller-Vedam LE, Navarro EJ, Palar A, Pellegrino J, Saylor W, Stephens CA, Strickland J, Torosyan H, Wankowicz SA, Wong DR, Wong G, Redding S, Chow ED, DeGrado WF, Kampmann M.
ACS Chem Biol. 2020 Aug 21;15(8):2137-2153. doi: 10.1021/acschembio.0c00339. Epub 2020 Aug 12.
PMID: 32786289 Free PMC article.
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Engineering and design: editorial overview.
Kuhlman B, DeGrado WF.
Curr Opin Struct Biol. 2009 Aug;19(4):440-1. doi: 10.1016/j.sbi.2009.07.010. Epub 2009 Aug 13.
PMID: 19683427 Free PMC article. No abstract available.
15
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beta-Peptides: from structure to function.
Cheng RP, Gellman SH, DeGrado WF.
Chem Rev. 2001 Oct;101(10):3219-32. doi: 10.1021/cr000045i.
PMID: 11710070 Review. No abstract available.
16
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Structural basis for integrin alphaIIbbeta3 clustering.
Li R, Bennett JS, Degrado WF.
Biochem Soc Trans. 2004 Jun;32(Pt3):412-5. doi: 10.1042/BST0320412.
PMID: 15157149 Review.
17
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Protein minimization: downsizing through mutation.
DeGrado WF, Sosnick TR.
Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5680-1. doi: 10.1073/pnas.93.12.5680.
PMID: 8650151 Free PMC article. Review. No abstract available.
18
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Protein design: a hierarchic approach.
Bryson JW, Betz SF, Lu HS, Suich DJ, Zhou HX, O'Neil KT, DeGrado WF.
Science. 1995 Nov 10;270(5238):935-41. doi: 10.1126/science.270.5238.935.
PMID: 7481798 Review.
19
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Protein design, a minimalist approach.
DeGrado WF, Wasserman ZR, Lear JD.
Science. 1989 Feb 3;243(4891):622-8. doi: 10.1126/science.2464850.
PMID: 2464850 Review.
20
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De novo designed synthetic mimics of antimicrobial peptides.
Scott RW, DeGrado WF, Tew GN.
Curr Opin Biotechnol. 2008 Dec;19(6):620-7. doi: 10.1016/j.copbio.2008.10.013. Epub 2008 Nov 17.
PMID: 18996193 Free PMC article. Review.
Page 3
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De novo design, synthesis and characterization of membrane-active peptides.
Lear JD, Gratkowski H, DeGrado WF.
Biochem Soc Trans. 2001 Aug;29(Pt 4):559-64. doi: 10.1042/bst0290559.
PMID: 11498028 Review.
Our current level of understanding of membrane-protein folding is primitive, but it is beginning to advance. Previously [Choma, Gratkowski, Lear and DeGrado (2000) Nat. Struct. Biol. 7, 161-166], we described studies of the association in detergent micelles of short, simpl …
22
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Design of self-assembling transmembrane helical bundles to elucidate principles required for membrane protein folding and ion transport.
Joh NH, Grigoryan G, Wu Y, DeGrado WF.
Philos Trans R Soc Lond B Biol Sci. 2017 Aug 5;372(1726):20160214. doi: 10.1098/rstb.2016.0214.
PMID: 28630154 Free PMC article. Review.
23
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Influenza A M2 Inhibitor Binding Understood through Mechanisms of Excess Proton Stabilization and Channel Dynamics.
Watkins LC, DeGrado WF, Voth GA.
J Am Chem Soc. 2020 Oct 14;142(41):17425-17433. doi: 10.1021/jacs.0c06419. Epub 2020 Sep 29.
PMID: 32933245 Free PMC article.
24
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Protein-directed self-assembly of a fullerene crystal.
Kim KH, Ko DK, Kim YT, Kim NH, Paul J, Zhang SQ, Murray CB, Acharya R, DeGrado WF, Kim YH, Grigoryan G.
Nat Commun. 2016 Apr 26;7:11429. doi: 10.1038/ncomms11429.
PMID: 27113637 Free PMC article.
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Foldamers as versatile frameworks for the design and evolution of function.
Goodman CM, Choi S, Shandler S, DeGrado WF.
Nat Chem Biol. 2007 May;3(5):252-62. doi: 10.1038/nchembio876.
PMID: 17438550 Free PMC article. Review.
26
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Protein-protein interactions in the membrane: sequence, structural, and biological motifs.
Moore DT, Berger BW, DeGrado WF.
Structure. 2008 Jul;16(7):991-1001. doi: 10.1016/j.str.2008.05.007.
PMID: 18611372 Free PMC article. Review.
27
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Modeling transmembrane helical oligomers.
Dieckmann GR, DeGrado WF.
Curr Opin Struct Biol. 1997 Aug;7(4):486-94. doi: 10.1016/s0959-440x(97)80111-x.
PMID: 9266169 Review.
28
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Peptide-Programmable Nanoparticle Superstructures with Tailored Electrocatalytic Activity.
Kang ES, Kim YT, Ko YS, Kim NH, Cho G, Huh YH, Kim JH, Nam J, Thach TT, Youn D, Kim YD, Yun WS, DeGrado WF, Kim SY, Hammond PT, Lee J, Kwon YU, Ha DH, Kim YH.
ACS Nano. 2018 Jul 24;12(7):6554-6562. doi: 10.1021/acsnano.8b01146. Epub 2018 Jun 4.
PMID: 29842775 Free PMC article.
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Structural basis for proton conduction and inhibition by the influenza M2 protein.
Hong M, DeGrado WF.
Protein Sci. 2012 Nov;21(11):1620-33. doi: 10.1002/pro.2158. Epub 2012 Oct 9.
PMID: 23001990 Free PMC article. Review.
30
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Structural and dynamic mechanisms for the function and inhibition of the M2 proton channel from influenza A virus.
Wang J, Qiu JX, Soto C, DeGrado WF.
Curr Opin Struct Biol. 2011 Feb;21(1):68-80. doi: 10.1016/j.sbi.2010.12.002. Epub 2011 Jan 17.
PMID: 21247754 Free PMC article. Review.
Page 4
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Designed metalloprotein stabilizes a semiquinone radical.
Ulas G, Lemmin T, Wu Y, Gassner GT, DeGrado WF.
Nat Chem. 2016 Apr;8(4):354-9. doi: 10.1038/nchem.2453. Epub 2016 Feb 15.
PMID: 27001731 Free PMC article.
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Native-like and structurally characterized designed alpha-helical bundles.
Betz SF, Bryson JW, DeGrado WF.
Curr Opin Struct Biol. 1995 Aug;5(4):457-63. doi: 10.1016/0959-440x(95)80029-8.
PMID: 8528761 Review.
33
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A long-lived Aß oligomer resistant to fibrillization.
Nick M, Wu Y, Schmidt NW, Prusiner SB, Stöhr J, DeGrado WF.
Biopolymers. 2018 Aug;109(8):e23096. doi: 10.1002/bip.23096. Epub 2018 Jan 10.
PMID: 29319162 Free PMC article.
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Self-assembling dipeptide antibacterial nanostructures with membrane disrupting activity.
Schnaider L, Brahmachari S, Schmidt NW, Mensa B, Shaham-Niv S, Bychenko D, Adler-Abramovich L, Shimon LJW, Kolusheva S, DeGrado WF, Gazit E.
Nat Commun. 2017 Nov 8;8(1):1365. doi: 10.1038/s41467-017-01447-x.
PMID: 29118336 Free PMC article.
35
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Folding of helical membrane proteins: the role of polar, GxxxG-like and proline motifs.
Senes A, Engel DE, DeGrado WF.
Curr Opin Struct Biol. 2004 Aug;14(4):465-79. doi: 10.1016/j.sbi.2004.07.007.
PMID: 15313242 Review.
36
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The accommodation index measures the perturbation associated with insertions and deletions in coiled-coils: Application to understand signaling in histidine kinases.
Schmidt NW, Grigoryan G, DeGrado WF.
Protein Sci. 2017 Mar;26(3):414-435. doi: 10.1002/pro.3095. Epub 2017 Feb 23.
PMID: 27977891 Free PMC article. Review.
37
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Designed peptides that assemble into cross-a amyloid-like structures.
Zhang SQ, Huang H, Yang J, Kratochvil HT, Lolicato M, Liu Y, Shu X, Liu L, DeGrado WF.
Nat Chem Biol. 2018 Sep;14(9):870-875. doi: 10.1038/s41589-018-0105-5. Epub 2018 Jul 30.
PMID: 30061717 Free PMC article.
38
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Blue fluorescent amino acid for biological spectroscopy and microscopy.
Hilaire MR, Ahmed IA, Lin CW, Jo H, DeGrado WF, Gai F.
Proc Natl Acad Sci U S A. 2017 Jun 6;114(23):6005-6009. doi: 10.1073/pnas.1705586114. Epub 2017 May 22.
PMID: 28533371 Free PMC article.
39
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Design of peptides and proteins.
Degrado WF.
Adv Protein Chem. 1988;39:51-124. doi: 10.1016/s0065-3233(08)60375-7.
PMID: 3072869 Review. No abstract available.
40
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X-ray Crystal Structures of the Influenza M2 Proton Channel Drug-Resistant V27A Mutant Bound to a Spiro-Adamantyl Amine Inhibitor Reveal the Mechanism of Adamantane Resistance.
Thomaston JL, Konstantinidi A, Liu L, Lambrinidis G, Tan J, Caffrey M, Wang J, Degrado WF, Kolocouris A.
Biochemistry. 2020 Feb 4;59(4):627-634. doi: 10.1021/acs.biochem.9b00971. Epub 2020 Jan 13.
PMID: 31894969 Free PMC article.
Page 5
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Proton-Induced Conformational and Hydration Dynamics in the Influenza A M2 Channel.
Watkins LC, Liang R, Swanson JMJ, DeGrado WF, Voth GA.
J Am Chem Soc. 2019 Jul 24;141(29):11667-11676. doi: 10.1021/jacs.9b05136. Epub 2019 Jul 12.
PMID: 31264413 Free PMC article.
42
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The twists and turns of beta-peptides.
DeGrado WF, Schneider JP, Hamuro Y.
J Pept Res. 1999 Sep;54(3):206-17. doi: 10.1034/j.1399-3011.1999.00131.x.
PMID: 10517158 Review.
43
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Modulating Integrin aIIbß3 Activity through Mutagenesis of Allosterically Regulated Intersubunit Contacts.
Tan SK, Fong KP, Polizzi NF, Sternisha A, Slusky JSG, Yoon K, DeGrado WF, Bennett JS.
Biochemistry. 2019 Jul 30;58(30):3251-3259. doi: 10.1021/acs.biochem.9b00430. Epub 2019 Jul 12.
PMID: 31264850 Free PMC article.
44
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Transmembrane communication: general principles and lessons from the structure and function of the M2 proton channel, K? channels, and integrin receptors.
Grigoryan G, Moore DT, DeGrado WF.
Annu Rev Biochem. 2011;80:211-37. doi: 10.1146/annurev-biochem-091008-152423.
PMID: 21548783 Free PMC article. Review.
45
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Genetically Introducing Biochemically Reactive Amino Acids Dehydroalanine and Dehydrobutyrine in Proteins.
Yang B, Wang N, Schnier PD, Zheng F, Zhu H, Polizzi NF, Ittuveetil A, Saikam V, DeGrado WF, Wang Q, Wang PG, Wang L.
J Am Chem Soc. 2019 May 15;141(19):7698-7703. doi: 10.1021/jacs.9b02611. Epub 2019 May 3.
PMID: 31038942 Free PMC article.
46
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De novo designed transmembrane peptides activating the a5ß1 integrin.
Mravic M, Hu H, Lu Z, Bennett JS, Sanders CR, Orr AW, DeGrado WF.
Protein Eng Des Sel. 2018 May 1;31(5):181-190. doi: 10.1093/protein/gzy014.
PMID: 29992271 Free PMC article.
47
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Synthesis and application of the blue fluorescent amino acid l-4-cyanotryptophan to assess peptide-membrane interactions.
Zhang K , Ahmed IA , Kratochvil HT , DeGrado WF , Gai F , Jo H .
Chem Commun (Camb). 2019 Apr 25;55(35):5095-5098. doi: 10.1039/c9cc01152h.
PMID: 30957824 Free PMC article.
48
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Computational biology: Biosensor design.
DeGrado WF.
Nature. 2003 May 8;423(6936):132-3. doi: 10.1038/423132a.
PMID: 12736670 No abstract available.
49
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De novo design and structural characterization of proteins and metalloproteins.
DeGrado WF, Summa CM, Pavone V, Nastri F, Lombardi A.
Annu Rev Biochem. 1999;68:779-819. doi: 10.1146/annurev.biochem.68.1.779.
PMID: 10872466 Review.
50
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4-Cyanoindole-2'-deoxyribonucleoside as a Dual Fluorescence and Infrared Probe of DNA Structure and Dynamics.
Ahmed IA, Acharyya A, Eng CM, Rodgers JM, DeGrado WF, Jo H, Gai F.
Molecules. 2019 Feb 8;24(3):602. doi: 10.3390/molecules24030602.
PMID: 30744004 Free PMC article.
Page 6
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Unique transmembrane domain interactions differentially modulate integrin avß3 and aIIbß3 function.
Litvinov RI, Mravic M, Zhu H, Weisel JW, DeGrado WF, Bennett JS.
Proc Natl Acad Sci U S A. 2019 Jun 18;116(25):12295-12300. doi: 10.1073/pnas.1904867116. Epub 2019 Jun 3.
PMID: 31160446 Free PMC article.
52
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Design of a switchable eliminase.
Korendovych IV, Kulp DW, Wu Y, Cheng H, Roder H, DeGrado WF.
Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):6823-7. doi: 10.1073/pnas.1018191108. Epub 2011 Apr 11.
PMID: 21482808 Free PMC article.
53
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Proteins from scratch.
DeGrado WF.
Science. 1997 Oct 3;278(5335):80-1. doi: 10.1126/science.278.5335.80.
PMID: 9340760 No abstract available.
54
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De Novo Design of Four-Helix Bundle Metalloproteins: One Scaffold, Diverse Reactivities.
Lombardi A, Pirro F, Maglio O, Chino M, DeGrado WF.
Acc Chem Res. 2019 May 21;52(5):1148-1159. doi: 10.1021/acs.accounts.8b00674. Epub 2019 Apr 11.
PMID: 30973707 Free PMC article.
55
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Zinc-binding structure of a catalytic amyloid from solid-state NMR.
Lee M, Wang T, Makhlynets OV, Wu Y, Polizzi NF, Wu H, Gosavi PM, Stöhr J, Korendovych IV, DeGrado WF, Hong M.
Proc Natl Acad Sci U S A. 2017 Jun 13;114(24):6191-6196. doi: 10.1073/pnas.1706179114. Epub 2017 May 31.
PMID: 28566494 Free PMC article.
56
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Packing of apolar side chains enables accurate design of highly stable membrane proteins.
Mravic M, Thomaston JL, Tucker M, Solomon PE, Liu L, DeGrado WF.
Science. 2019 Mar 29;363(6434):1418-1423. doi: 10.1126/science.aav7541.
PMID: 30923216 Free PMC article.
57
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Aß and tau prion-like activities decline with longevity in the Alzheimer's disease human brain.
Aoyagi A, Condello C, Stöhr J, Yue W, Rivera BM, Lee JC, Woerman AL, Halliday G, van Duinen S, Ingelsson M, Lannfelt L, Graff C, Bird TD, Keene CD, Seeley WW, DeGrado WF, Prusiner SB.
Sci Transl Med. 2019 May 1;11(490):eaat8462. doi: 10.1126/scitranslmed.aat8462.
PMID: 31043574 Free PMC article.
58
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Constructing ion channels from water-soluble a-helical barrels.
Scott AJ, Niitsu A, Kratochvil HT, Lang EJM, Sengel JT, Dawson WM, Mahendran KR, Mravic M, Thomson AR, Brady RL, Liu L, Mulholland AJ, Bayley H, DeGrado WF, Wallace MI, Woolfson DN.
Nat Chem. 2021 Jul;13(7):643-650. doi: 10.1038/s41557-021-00688-0. Epub 2021 May 10.
PMID: 33972753 Free PMC article.
59
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How calmodulin binds its targets: sequence independent recognition of amphiphilic alpha-helices.
O'Neil KT, DeGrado WF.
Trends Biochem Sci. 1990 Feb;15(2):59-64. doi: 10.1016/0968-0004(90)90177-d.
PMID: 2186516 Review.
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Exposing the Nucleation Site in a-Helix Folding: A Joint Experimental and Simulation Study.
Acharyya A, Ge Y, Wu H, DeGrado WF, Voelz VA, Gai F.
J Phys Chem B. 2019 Feb 28;123(8):1797-1807. doi: 10.1021/acs.jpcb.8b12220. Epub 2019 Feb 14.
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Photoactivatable protein labeling by singlet oxygen mediated reactions.
To TL, Medzihradszky KF, Burlingame AL, DeGrado WF, Jo H, Shu X.
Bioorg Med Chem Lett. 2016 Jul 15;26(14):3359-3363. doi: 10.1016/j.bmcl.2016.05.034. Epub 2016 May 12.
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Graphene Symmetry Amplified by Designed Peptide Self-Assembly.
Mustata GM, Kim YH, Zhang J, DeGrado WF, Grigoryan G, Wanunu M.
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De novo design of helical bundles as models for understanding protein folding and function.
Hill RB, Raleigh DP, Lombardi A, DeGrado WF.
Acc Chem Res. 2000 Nov;33(11):745-54. doi: 10.1021/ar970004h.
PMID: 11087311 Free PMC article. Review.
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How do helix-helix interactions help determine the folds of membrane proteins? Perspectives from the study of homo-oligomeric helical bundles.
DeGrado WF, Gratkowski H, Lear JD.
Protein Sci. 2003 Apr;12(4):647-65. doi: 10.1110/ps.0236503.
PMID: 12649422 Free PMC article. Review.
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Nature-inspired design of motif-specific antibody scaffolds.
Koerber JT, Thomsen ND, Hannigan BT, Degrado WF, Wells JA.
Nat Biotechnol. 2013 Oct;31(10):916-21. doi: 10.1038/nbt.2672. Epub 2013 Aug 18.
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Computational design of a protein crystal.
Lanci CJ, MacDermaid CM, Kang SG, Acharya R, North B, Yang X, Qiu XJ, DeGrado WF, Saven JG.
Proc Natl Acad Sci U S A. 2012 May 8;109(19):7304-9. doi: 10.1073/pnas.1112595109. Epub 2012 Apr 25.
PMID: 22538812 Free PMC article.
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Short peptides self-assemble to produce catalytic amyloids.
Rufo CM, Moroz YS, Moroz OV, Stöhr J, Smith TA, Hu X, DeGrado WF, Korendovych IV.
Nat Chem. 2014 Apr;6(4):303-9. doi: 10.1038/nchem.1894. Epub 2014 Mar 16.
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High-density grids for efficient data collection from multiple crystals.
Baxter EL, Aguila L, Alonso-Mori R, Barnes CO, Bonagura CA, Brehmer W, Brunger AT, Calero G, Caradoc-Davies TT, Chatterjee R, Degrado WF, Fraser JS, Ibrahim M, Kern J, Kobilka BK, Kruse AC, Larsson KM, Lemke HT, Lyubimov AY, Manglik A, McPhillips SE, Norgren E, Pang SS, Soltis SM, Song J, Thomaston J, Tsai Y, Weis WI, Woldeyes RA, Yachandra V, Yano J, Zouni A, Cohen AE.
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Glutamine Side Chain 13C-18O as a Nonperturbative IR Probe of Amyloid Fibril Hydration and Assembly.
Wu H, Saltzberg DJ, Kratochvil HT, Jo H, Sali A, DeGrado WF.
J Am Chem Soc. 2019 May 8;141(18):7320-7326. doi: 10.1021/jacs.9b00577. Epub 2019 Apr 24.
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Crystal structure of the drug-resistant S31N influenza M2 proton channel.
Thomaston JL, DeGrado WF.
Protein Sci. 2016 Aug;25(8):1551-4. doi: 10.1002/pro.2937. Epub 2016 May 17.
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Salt bridges: geometrically specific, designable interactions.
Donald JE, Kulp DW, DeGrado WF.
Proteins. 2011 Mar;79(3):898-915. doi: 10.1002/prot.22927. Epub 2011 Jan 5.
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A 31-residue peptide induces aggregation of tau's microtubule-binding region in cells.
Stöhr J, Wu H, Nick M, Wu Y, Bhate M, Condello C, Johnson N, Rodgers J, Lemmin T, Acharya S, Becker J, Robinson K, Kelly MJS, Gai F, Stubbs G, Prusiner SB, DeGrado WF.
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Small molecule inhibitors of integrin alpha2beta1.
Choi S, Vilaire G, Marcinkiewicz C, Winkler JD, Bennett JS, DeGrado WF.
J Med Chem. 2007 Nov 1;50(22):5457-62. doi: 10.1021/jm070252b. Epub 2007 Oct 4.
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Tertiary templates for the design of diiron proteins.
Summa CM, Lombardi A, Lewis M, DeGrado WF.
Curr Opin Struct Biol. 1999 Aug;9(4):500-8. doi: 10.1016/S0959-440X(99)80071-2.
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Design of a Short Thermally Stable a-Helix Embedded in a Macrocycle.
Wu H, Acharyya A, Wu Y, Liu L, Jo H, Gai F, DeGrado WF.
Chembiochem. 2018 May 4;19(9):902-906. doi: 10.1002/cbic.201800026. Epub 2018 Mar 22.
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Amphiphilic polymethacrylate derivatives as antimicrobial agents.
Kuroda K, DeGrado WF.
J Am Chem Soc. 2005 Mar 30;127(12):4128-9. doi: 10.1021/ja044205+.
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De novo design of a hyperstable non-natural protein-ligand complex with sub-Å accuracy.
Polizzi NF, Wu Y, Lemmin T, Maxwell AM, Zhang SQ, Rawson J, Beratan DN, Therien MJ, DeGrado WF.
Nat Chem. 2017 Dec;9(12):1157-1164. doi: 10.1038/nchem.2846. Epub 2017 Aug 21.
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De novo design of a transmembrane Zn²?-transporting four-helix bundle.
Joh NH, Wang T, Bhate MP, Acharya R, Wu Y, Grabe M, Hong M, Grigoryan G, DeGrado WF.
Science. 2014 Dec 19;346(6216):1520-4. doi: 10.1126/science.1261172.
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X-ray Crystal Structure of the Influenza A M2 Proton Channel S31N Mutant in Two Conformational States: An Open and Shut Case.
Thomaston JL, Wu Y, Polizzi N, Liu L, Wang J, DeGrado WF.
J Am Chem Soc. 2019 Jul 24;141(29):11481-11488. doi: 10.1021/jacs.9b02196. Epub 2019 Jul 11.
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De novo design of catalytic proteins.
Kaplan J, DeGrado WF.
Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11566-70. doi: 10.1073/pnas.0404387101. Epub 2004 Aug 3.
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A real-time all-atom structural search engine for proteins.
Gonzalez G, Hannigan B, DeGrado WF.
PLoS Comput Biol. 2014 Jul 31;10(7):e1003750. doi: 10.1371/journal.pcbi.1003750. eCollection 2014 Jul.
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De Novo Design, Solution Characterization, and Crystallographic Structure of an Abiological Mn-Porphyrin-Binding Protein Capable of Stabilizing a Mn(V) Species.
Mann SI, Nayak A, Gassner GT, Therien MJ, DeGrado WF.
J Am Chem Soc. 2021 Jan 13;143(1):252-259. doi: 10.1021/jacs.0c10136. Epub 2020 Dec 29.
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In vitro 0N4R tau fibrils contain a monomorphic ß-sheet core enclosed by dynamically heterogeneous fuzzy coat segments.
Dregni AJ, Mandala VS, Wu H, Elkins MR, Wang HK, Hung I, DeGrado WF, Hong M.
Proc Natl Acad Sci U S A. 2019 Aug 13;116(33):16357-16366. doi: 10.1073/pnas.1906839116. Epub 2019 Jul 29.
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Computational design of a self-assembling ß-peptide oligomer.
Korendovych IV, Kim YH, Ryan AH, Lear JD, Degrado WF, Shandler SJ.
Org Lett. 2010 Nov 19;12(22):5142-5. doi: 10.1021/ol102092r. Epub 2010 Oct 14.
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Proximity-enhanced SuFEx chemical cross-linker for specific and multitargeting cross-linking mass spectrometry.
Yang B, Wu H, Schnier PD, Liu Y, Liu J, Wang N, DeGrado WF, Wang L.
Proc Natl Acad Sci U S A. 2018 Oct 30;115(44):11162-11167. doi: 10.1073/pnas.1813574115. Epub 2018 Oct 15.
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Inhibition of integrin a2ß1 ameliorates glomerular injury.
Borza CM, Su Y, Chen X, Yu L, Mont S, Chetyrkin S, Voziyan P, Hudson BG, Billings PC, Jo H, Bennett JS, Degrado WF, Eckes B, Zent R, Pozzi A.
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Computational design of a ß-peptide that targets transmembrane helices.
Shandler SJ, Korendovych IV, Moore DT, Smith-Dupont KB, Streu CN, Litvinov RI, Billings PC, Gai F, Bennett JS, DeGrado WF.
J Am Chem Soc. 2011 Aug 17;133(32):12378-81. doi: 10.1021/ja204215f. Epub 2011 Jul 22.
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Antibacterial mechanism of action of arylamide foldamers.
Mensa B, Kim YH, Choi S, Scott R, Caputo GA, DeGrado WF.
Antimicrob Agents Chemother. 2011 Nov;55(11):5043-53. doi: 10.1128/AAC.05009-11. Epub 2011 Aug 15.
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Analysis and design of three-stranded coiled coils and three-helix bundles.
Schneider JP, Lombardi A, DeGrado WF.
Fold Des. 1998;3(2):R29-40. doi: 10.1016/S1359-0278(98)00011-X.
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Exploring N-Arylsulfonyl-l-proline Scaffold as a Platform for Potent and Selective avß1 Integrin Inhibitors.
Reed NI, Tang YZ, McIntosh J, Wu Y, Molnar KS, Civitavecchia A, Sheppard D, DeGrado WF, Jo H.
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Acid activation mechanism of the influenza A M2 proton channel.
Liang R, Swanson JMJ, Madsen JJ, Hong M, DeGrado WF, Voth GA.
Proc Natl Acad Sci U S A. 2016 Nov 8;113(45):E6955-E6964. doi: 10.1073/pnas.1615471113. Epub 2016 Oct 24.
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An assay suitable for high throughput screening of anti-influenza drugs.
Mao L, Wang J, DeGrado WF, Inouye M.
PLoS One. 2013;8(1):e54070. doi: 10.1371/journal.pone.0054070. Epub 2013 Jan 10.
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Light-induced helix formation.
Huang CY, He S, DeGrado WF, McCafferty DG, Gai F.
J Am Chem Soc. 2002 Oct 30;124(43):12674-5. doi: 10.1021/ja028084u.
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Pharmacologic Blockade of avß1 Integrin Ameliorates Renal Failure and Fibrosis In Vivo.
Chang Y, Lau WL, Jo H, Tsujino K, Gewin L, Reed NI, Atakilit A, Nunes ACF, DeGrado WF, Sheppard D.
J Am Soc Nephrol. 2017 Jul;28(7):1998-2005. doi: 10.1681/ASN.2015050585. Epub 2017 Feb 20.
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Crystallization of proton channel peptides.
Lovejoy B, Akerfeldt KS, DeGrado WF, Eisenberg D.
Protein Sci. 1992 Aug;1(8):1073-7. doi: 10.1002/pro.5560010812.
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De novo design of covalently constrained mesosize protein scaffolds with unique tertiary structures.
Dang B, Wu H, Mulligan VK, Mravic M, Wu Y, Lemmin T, Ford A, Silva DA, Baker D, DeGrado WF.
Proc Natl Acad Sci U S A. 2017 Oct 10;114(41):10852-10857. doi: 10.1073/pnas.1710695114. Epub 2017 Sep 25.
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Artificial diiron proteins: from structure to function.
Calhoun JR, Nastri F, Maglio O, Pavone V, Lombardi A, DeGrado WF.
Biopolymers. 2005;80(2-3):264-78. doi: 10.1002/bip.20230.
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Peptide engineering. Catalytic molten globules.
DeGrado WF.
Nature. 1993 Oct 7;365(6446):488-9. doi: 10.1038/365488a0.
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Spectroscopic and metal binding properties of a de novo metalloprotein binding a tetrazinc cluster.
Chino M, Zhang SQ, Pirro F, Leone L, Maglio O, Lombardi A, DeGrado WF.
Biopolymers. 2018 Aug;109(10):e23339. doi: 10.1002/bip.23229. Epub 2018 Sep 11.
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Polar networks control oligomeric assembly in membranes.
Tatko CD, Nanda V, Lear JD, Degrado WF.
J Am Chem Soc. 2006 Apr 5;128(13):4170-1. doi: 10.1021/ja055561a.
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